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JoVE Journal Neuroscience
I Vivo blod - hjerne barrieren permeabilitet analysen mus med Fluorescently merket Tracers
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I Vivo blod - hjerne barrieren permeabilitet analysen mus med Fluorescently merket Tracers

Article DOI: 10.3791/57038-v • 09:35 min • February 26th, 2018
February 26th, 2018 •
1,2, 1, 1, 1
1Institute of Neurology (Edinger Institute), Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital

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Her presenterer vi en mus hjernen vaskulær permeabilitet analysen bruker intraperitoneal injeksjon av fluorescerende tracers etterfulgt av perfusjon som gjelder for dyr modeller av blod - hjerne barrieren dysfunksjon. En hemi-hjerne er brukt for å vurdere permeabilitet kvantitativt, og den andre for tracer visualisering/immunostaining. Prosedyren tar 5-6 h for 10 mus.

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Nevrovitenskap problemet 132 blod - hjerne barrieren BBB i vivo permeabilitet kvantitativ fluorescerende tracers endotelceller microvessels kapillærene intraperitoneal perfusjon
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