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Journal / Developmental Biology / Genetische Technik der primären…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Genetische Technik der primären Maus-Darm-Organoide mit magnetischen Nanopartikel-Transduktion virale Vektoren für gefrorene Schnitte
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Genetische Technik der primären Maus-Darm-Organoide mit magnetischen Nanopartikel-Transduktion virale Vektoren für gefrorene Schnitte

Article DOI: 10.3791/57040-v • 07:37 min • May 10th, 2019
May 10th, 2019 •
1, 1,5, 2, 1, 1, 3,4, 1,4,5,6
1Department of Medicine, Division of Hematology, Johns Hopkins University School of Medicine, 2Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, 3Department of Cell Biology, Johns Hopkins University School of Medicine, 4Department of Oncology, Johns Hopkins University School of Medicine, 5Department of Pathology, Johns Hopkins University School of Medicine, 6Institute for Cell Engineering, Johns Hopkins University School of Medicine

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Wir beschreiben Schritt-für-Schritt-Anleitungen zu: 1) effiziente Technische Darmorganoide mit magnetischen Nanopartikeln für lenti- oder retrovirale Transduktion, und, 2) erzeugen gefrorene Abschnitte aus technischen Organoiden. Dieser Ansatz bietet ein leistungsfähiges Werkzeug, um die Genexpression in Organoiden effizient zu verändern, um nachgelagerte Effekte zu untersuchen.

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Dieser Monat in JoVE Ausgabe 147 Lentivirus Maus Darm Organoide Magnet Nanopartikel Retrovirus
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