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Journal / Developmental Biology / Genetisk engineering av primær mus intestinal…
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Developmental Biology

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JoVE Journal Developmental Biology
Genetisk engineering av primær mus intestinal Organoids bruke magnetisk Nanopartikkel Transduction viral vektorer for frossen snitting
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Genetisk engineering av primær mus intestinal Organoids bruke magnetisk Nanopartikkel Transduction viral vektorer for frossen snitting

Article DOI: 10.3791/57040-v • 07:37 min • May 10th, 2019
May 10th, 2019 •
1, 1,5, 2, 1, 1, 3,4, 1,4,5,6
1Department of Medicine, Division of Hematology, Johns Hopkins University School of Medicine, 2Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, 3Department of Cell Biology, Johns Hopkins University School of Medicine, 4Department of Oncology, Johns Hopkins University School of Medicine, 5Department of Pathology, Johns Hopkins University School of Medicine, 6Institute for Cell Engineering, Johns Hopkins University School of Medicine

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Vi beskriver trinnvise instruksjoner til: 1) effektivt ingeniør intestinal organoids ved hjelp av magnetiske nanopartikler for Lenti-eller retroviral Transduction, og, 2) generere frosne seksjoner fra konstruert organoids. Denne tilnærmingen gir et kraftig verktøy for å effektivt endre genuttrykk i organoids for etterforskning av nedstrøms effekter.

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Denne måneden i JoVE lentivirus mus tarm Organoids magnetisk nanopartikler retrovirus
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