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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
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Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Article DOI: 10.3791/57042-v • 12:04 min • May 9th, 2018
May 9th, 2018 •
*1, *1, 1, 1, 1, 2, 2, 3, 4, 4, 5, 1,6, 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
* These authors contributed equally

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Summary
Automatic Translation

In view of the 3Rs principle, respiratory models as alternatives to animal studies are evolving. Especially for risk assessment of respiratory substances, there is a lack of appropriate assays. Here, we describe the use of human precision-cut lung slices for the assessment of airborne substances.

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Human Precision-cut Lung Slices Cytotoxicity Immunomodulatory Effects Ex Vivo Model Pharmacology Toxicology Safety Evaluation Physiological Response Respiratory Diseases Fresh Human Tissue Human Cell-cell Interactions Pathomechanism Respiratory Disease Species-specific Applications Reliable Infrastructure Cannulation Of Trachea Silicone Tube Clamp Fixation
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