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Journal / Developmental Biology / Flere hierarkisk Imaging av føljetong delene for…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Flere hierarkisk Imaging av føljetong delene for å finne konkrete mobilnettet mål i store volumer
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Flere hierarkisk Imaging av føljetong delene for å finne konkrete mobilnettet mål i store volumer

Article DOI: 10.3791/57059-v • 11:19 min • March 20th, 2018
March 20th, 2018 •
1,2, 3, 2,4, 2,4, 5, 6, 2,4, 1,2,3
1Cryo Electron Microscopy, Centre for Advanced Materials, Universität Heidelberg, 2Heidelberg Karlsruhe Research Partnership (HEiKA), 3Cryo Electron Microscopy, BioQuant, Universitätsklinikum Heidelberg, 4Institute for Automation and Applied Computer Science, Karlsruhe Institute of Technology (KIT), 5Carl Zeiss Microscopy GmbH, 6Electron Microscopy Core Facility, Universität Heidelberg

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Denne protokollen mål bestemte celler i vev for bildebehandling nanoskala oppløsning bruker en scanning elektron mikroskop (SEM). Stort antall serielle inndelinger fra harpiks-innebygd biologisk materiale er først fotografert i en lys mikroskop å identifisere målet og deretter i en hierarkisk måte i SEM.

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Utviklingsbiologi problemet 133 matrise tomografi store volum elektronmikroskop skanning elektronmikroskop fluorescens * lys korrelert lys og elektronmikroskop CLEM hierarkisk tenkelig automatisert imaging målretting
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