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JoVE Journal
Biology

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甘蓝型油菜中提取3页蛋白质和核糖配合物的韧皮部汁液
 
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甘蓝型油菜中提取3页蛋白质和核糖配合物的韧皮部汁液

Article DOI: 10.3791/57097-v 11:23 min January 9th, 2018
January 9th, 2018

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Summary

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在这里, 我们提出了一个协议, 以分析蛋白质组成的大本地蛋白质: 蛋白质和蛋白质: 核酸复合物油菜 (B. 油菜)韧皮部渗出液使用3D 聚丙烯酰胺凝胶电泳 (PAGE) 方法结合蓝色本机 (BN) 与两个变性页后, 质谱鉴定。

Tags

分子生物学 问题 131 蓝色本机页 韧皮部汁液抽样 蛋白质复合体 核糖复合物 质谱 甘蓝型油菜
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