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Muestreo de Sap de líber de Brassica napus para 3D-página de proteínas y complejos de ribonucleoproteína
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Muestreo de Sap de líber de Brassica napus para 3D-página de proteínas y complejos de ribonucleoproteína

Article DOI: 10.3791/57097-v • 11:23 min • January 9th, 2018
January 9th, 2018 •
1, 1, 1, 1
1Molecular Plant Genetics, Universität Hamburg

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Aquí presentamos un protocolo para analizar la composición de proteína de proteína: proteína nativa grande y proteínas: complejos de ácido nucleico de colza (B. napus) exudado líber utilizando un enfoque 3D poliacrilamida gel electroforesis (PAGE) combinando azul nativo (BN) con dos páginas desnaturalización seguida de identificación espectrométrica de masa.

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Biología molecular número 131 página nativa azul líber savia muestreo complejo de proteínas espectrometría de masa complejo de ribonucleoproteína Brassica napus
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