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Journal / Immunology and Infection / 해 다중 단백질 신호 복합물 분자 보완성 친 화력 정화 (BiCAP)에…
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Immunology and Infection

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JoVE Journal Immunology and Infection
해 다중 단백질 신호 복합물 분자 보완성 친 화력 정화 (BiCAP)에 의해
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
Journal (Immunology and Infection)
Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants
High-Throughput Screening of Compounds Using Neutrophils Isolated from Human Blood in a 384-Well Plate Format (Video) | JoVE
Journal (Immunology and Infection)
High-Throughput Screening of Compounds Using Neutrophils Isolated from Human Blood in a 384-Well Plate Format (Video) | JoVE
 
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해 다중 단백질 신호 복합물 분자 보완성 친 화력 정화 (BiCAP)에 의해

Article DOI: 10.3791/57109-v • 06:45 min • June 15th, 2018
June 15th, 2018 •
1, 1, 1,2, 1,3, 1,4, 5, 1,6,7
1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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이 원고는 분자 보완성 친 화력 정화 (BiCAP)에 대 한 프로토콜을 설명합니다. 이 새로운 방법은 경쟁 바인딩 파트너와 함께 형성 된 복합물 뿐만 아니라 유엔 complexed 개별 단백질을 제외 하는 동안 특정 격리 및 어떤 두 상호 작용 단백질의 다운스트림 proteomic 특성화를 촉진 한다.

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면역학 그리고 감염 문제 136 단백질 단백질 상호 작용 Proteomics 상호작용체학 BiCAP 신호 변환 분자 형광 보완성 Nanobody
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