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Journal / Immunology and Infection / 针对天然产物的单克隆抗体的产生
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Immunology and Infection
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JoVE Journal Immunology and Infection
针对天然产物的单克隆抗体的产生
A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
Journal (Immunology and Infection)
A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
Journal (Immunology and Infection)
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
 
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针对天然产物的单克隆抗体的产生

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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本文提供了一个详细的方案, 用于制备和评价用于各种免疫检测的天然产品单克隆抗体。该程序包括免疫接种、细胞融合、用于阳性克隆筛选的间接竞争性 ELISA 和单克隆杂交瘤制剂。还提供了使用 maldi TOF-MS 和 ELISA 分析进行抗体表征的规范。

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免疫学与感染 第146期 单克隆抗体、人工抗原、天然产物、小分子化合物、免疫、杂交瘤
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