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Journal / Immunology and Infection / Generation av monoklonala antikroppar mot…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation av monoklonala antikroppar mot naturliga produkter
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Generation av monoklonala antikroppar mot naturliga produkter

Article DOI: 10.3791/57116-v • 12:15 min • April 6th, 2019
April 6th, 2019 •
1, 2, 3, 4, 3, 3, 5
1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine

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Den här artikeln innehåller ett detaljerat protokoll för utarbetande och utvärdering av monoklonala antikroppar mot naturliga produkter för användning i olika immunanalyser. Denna procedur innefattar immunisering, Cellfusion, indirekta kompetitiva ELISA-test för positiva klon screening och monoklonala Hybridomteknik förberedelse. Specifikationerna för antikropp karakterisering med MALDI-TOF-MS och ELISA analyser finns också.

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Immunologi och infektion fråga 146 monoklonal antikropp konstgjorda antigen naturen produkt små molekylära förening immunisering hybridom
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