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Journal / Chemistry / Chip microfluidici per dispersione della luce…
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Chemistry

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JoVE Journal Chemistry
Chip microfluidici per dispersione della luce dinamica In Situ diffrazione di raggi x di cristallo ed In Situ per la cristallografia seriale
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Chip microfluidici per dispersione della luce dinamica In Situ diffrazione di raggi x di cristallo ed In Situ per la cristallografia seriale

Article DOI: 10.3791/57133-v • 11:48 min • April 24th, 2018
April 24th, 2018 •
*1,2, *3,4,5, 3, 6, 6, 3,4, 3,4,5, 1,2,4, 1,7
1Center for Free Electron Laser Science, DESY, 2Department of Physics, University of Hamburg, 3Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, 4The Hamburg Center for Ultrafast Imaging, University of Hamburg, 5Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI), 6European Molecular Biology Laboratory, EMBL c/o DESY, 7Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
* These authors contributed equally

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Questo protocollo descrive dettagliatamente come fabbricare e gestire dispositivi microfluidici per la raccolta di dati di diffrazione di raggi x a temperatura ambiente. Inoltre, viene descritto come monitorare la cristallizzazione della proteina di dispersione della luce dinamica e come elaborare e analizzare dati di diffrazione ottenuti.

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Chimica problema 134 in situ diffrazione di raggi x fissato target cristallografia seriale millisecondo microfluidica cristallizzazione di proteine in situ dynamic light scattering
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