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En Optogenetic metode til at styre og analysere gen Expression mønstre i celle-til-celle interaktioner

Article DOI: 10.3791/57149-v • 07:59 min • March 22nd, 2018
March 22nd, 2018 •
1,2, 1,3,4,5
1Institute for Frontier Life and Medical Sciences, Kyoto University, 2Japan Science and Technology Agency, PRESTO, 3Institute for Integrated Cell-Material Sciences, Kyoto University, 4Graduate School of Medicine, Kyoto University, 5Graduate School of Biostudies, Kyoto University

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Vi præsenterer her, en protokol for at analysere celle til celle overførsel af oscillerende oplysninger optogenetic kontrol og live overvågning af genekspression. Denne fremgangsmåde giver en enestående platform for at teste en funktionel betydning af dynamiske gen expression programmer i flercellede systemer.

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Genetik sag 133 optogenetic metode real-time imaging luciferase reporter GAVPO oscillerende udtryk Notch signalering
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