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Bioengineering

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JoVE Journal Bioengineering
Isolamento de células-tronco mesenquimais do periósteo Alveolar humano e efeitos da vitamina D na atividade osteogênica do periósteo-derivado de células
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Isolamento de células-tronco mesenquimais do periósteo Alveolar humano e efeitos da vitamina D na atividade osteogênica do periósteo-derivado de células

Article DOI: 10.3791/57166-v • 06:47 min • May 4th, 2018
May 4th, 2018 •
1,2, 3, 1,4,5,6, 1,7,8
1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

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Apresentamos um protocolo para investigar os biomarcadores de expressão do mRNA de células periósteo-derivado (PDCs) induzidas pela vitamina C (vitamina C) e 1,25-dihidroxi vitamina D [1,25-(OH)2D3]. Além disso, avaliamos a capacidade dos PDCs para diferenciar em adipócitos, condrócitos e osteócitos.

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Bioengenharia edição 135 periósteo Alveolar células-tronco mesenquimais 1,25-(OH)2 D3 vitamina D3 Calcitriol periósteo
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