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JoVE Journal Bioengineering
Isolamento di cellule staminali mesenchimali da periostio alveolare umano e gli effetti della vitamina D su attività osteogenica delle cellule derivate dal periostio
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Isolamento di cellule staminali mesenchimali da periostio alveolare umano e gli effetti della vitamina D su attività osteogenica delle cellule derivate dal periostio

Article DOI: 10.3791/57166-v • 06:47 min • May 4th, 2018
May 4th, 2018 •
1,2, 3, 1,4,5,6, 1,7,8
1Chang Gung University, 2Department of Periodontics, Chang Gung Memorial Hospital, 3California Northstate University College of Medicine, 4Department of Nephrology, Clinical Poison Center, Chang Gung Memorial Hospital, 5Kidney Research Center, Chang Gung Memorial Hospital, 6Center for Tissue Engineering, Chang Gung Memorial Hospital, 7Department of Periodontics, Chang Gung Memorial Hospital, 8College of Oral Medicine, Taipei Medical University

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Vi presentiamo un protocollo per studiare i biomarcatori di espressione di mRNA di cellule derivanti dal periostio (PDC) indotte da vitamina C (vitamina C) e 1,25-diidrossi-vitamina D [1,252D-3]. Inoltre, valutiamo la capacità di differenziarsi in adipociti, condrociti e osteociti PDC.

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Bioingegneria emissione 135 periostio alveolare cellule staminali mesenchimali 1,252D-3 vitamina D3 Calcitriol periostio
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