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Journal / Chemistry / Essentieel metalen opname bij gram-negatieve…
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Chemistry

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JoVE Journal Chemistry
Essentieel metalen opname bij gram-negatieve bacteriën
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Essentieel metalen opname bij gram-negatieve bacteriën: Röntgen fluorescentie, radio-isotopen, en cel fractionering

Article DOI: 10.3791/57169-v • 10:34 min • February 1st, 2018
February 1st, 2018 •
1, 2, 2, 3, 2, 4
1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

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Een protocol voor de winning van een chaperone periplasmische overgangsmetalen in het kader van haar eigen bindende partners, en biofysische karakterisering van de inhoud van het substraat door Röntgen fluorescentie en radiometal opname wordt gepresenteerd.

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Chemie kwestie 132 fractionering periplasma Röntgen fluorescentie overgangsmetalen substraat-bindende eiwit (SBP) YfeA Yersinia pestis pest Radiotracer radioactiviteit
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