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Inclusione in paraffina e sezionamento sottile di biofilm di colonie microbiche per l'analisi al microscopio

Article DOI: 10.3791/57196-v • 08:52 min • March 23rd, 2018
March 23rd, 2018 •
1, 1, 1,2, 1, 2, 1
1Department of Biological Sciences, Columbia University, 2Department of Biology, Barnard College, Columbia University

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Descriviamo la fissazione, inclusione in paraffina e sottili tecniche di sezionamento per colonie microbiche biofilm. In campioni preparati, modelli di espressione di sottostruttura e reporter di biofilm possono essere visualizzati da microscopia.

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Inclusione Colonia Biofilm la fissazione del paraformaldeide fluorescenza microscopia macchia di Lectin Pel polisaccaride Biofilm Matrix a sezione sottile Biofilm paraffina immunologia e infezione problema 133,
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