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Journal / Biology / Visualisering av DNA komprimering i Cyanobakterie …
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Biology

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Visualisering av DNA komprimering i Cyanobakterie av høyspent Cryo-elektron tomografi
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Visualisering av DNA komprimering i Cyanobakterie av høyspent Cryo-elektron tomografi

Article DOI: 10.3791/57197-v • 09:47 min • July 17th, 2018
July 17th, 2018 •
1, 2
1National Institute for Physiological Sciences, 2Graduate School of Science and Engineering, Saitama University

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Denne protokollen beskriver hvordan å visualisere forbigående DNA komprimeringsprosessen i Cyanobakterie. Synkron dyrking, overvåking av fluorescens mikroskopi, frysing og høy spenning cryo-elektron tomografi brukes. En protokoll for disse metodene er presentert, og fremtidige programmer og utviklingen er diskutert.

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Biologi problemet 137 synkron kultur fluorescens mikroskopi rask frysing høyspent cryo-elektron tomografi segmentering Cyanobakterie DNA komprimering celledeling tykk prøven
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