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Developmental Biology

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JoVE Journal Developmental Biology
Identificazione delle cellule satelliti del muscolo scheletrico dall'immunofluorescenza con Pax7 e Laminin anticorpi
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Identificazione delle cellule satelliti del muscolo scheletrico dall'immunofluorescenza con Pax7 e Laminin anticorpi

Article DOI: 10.3791/57212-v • 07:18 min • April 19th, 2018
April 19th, 2018 •
*1, *2, 1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health
* These authors contributed equally

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La precisa identificazione delle cellule satelliti è essenziale per lo studio delle loro funzioni in varie condizioni fisiologiche e patologiche. Questo articolo presenta un protocollo per identificare cellule satelliti su sezioni del muscolo scheletrico adulto tramite basati su immunofluorescenza colorazione.

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Biologia dello sviluppo problema 134 muscolo scheletrico cellule satelliti Pax7 immunofluorescenza cellule staminali lamina basale
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