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Journal / Developmental Biology / Identifiering av skelettmuskulaturen satellit…
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Developmental Biology

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JoVE Journal Developmental Biology
Identifiering av skelettmuskulaturen satellit celler genom immunofluorescens med Pax7 och Laminin antikroppar
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Identifiering av skelettmuskulaturen satellit celler genom immunofluorescens med Pax7 och Laminin antikroppar

Article DOI: 10.3791/57212-v • 07:18 min • April 19th, 2018
April 19th, 2018 •
*1, *2, 1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health
* These authors contributed equally

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Exakt identifiering av satellit celler är viktigt för att studera deras funktioner under olika fysiologiska och patologiska förhållanden. Denna artikel presenterar ett protokoll för att identifiera satellit celler på vuxen skelettmuskulatur sektioner av immunofluorescens-baserade färgning.

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Utvecklingsbiologi fråga 134 skelettmuskel satellit celler Pax7 immunofluorescens stamceller basala lamina
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