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Journal / Neuroscience / Combining Optogenetics with Artificial microRNAs…
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Neuroscience

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JoVE Journal Neuroscience
Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
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Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits

Article DOI: 10.3791/57223-v • 09:17 min • March 14th, 2018
March 14th, 2018 •
1, 1, 1
1Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia

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Summary
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This protocol provides a workflow on how to combine artificial microRNA-mediated RNA interference with optogenetics to stimulate specifically presynaptic boutons with reduced expression of selective gene(s) within intact neuronal circuits.

Tags

Optogenetics Artificial MicroRNAs Gene Knockdown Presynaptic Function Neuronal Circuits RNA Interference Synaptic Physiology Presynaptic Proteins Synaptic Transmission Brain Circuits Acute Brain Slices Neuronal Cultures Corticoculturing Protocol
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