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Biochemistry

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JoVE Journal Biochemistry
Lipid indeks bestemmelse af flydende fluorescens opsving i svampe patogen Ustilago Maydis
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Lipid indeks bestemmelse af flydende fluorescens opsving i svampe patogen Ustilago Maydis

Article DOI: 10.3791/57279-v • 10:22 min • April 3rd, 2018
April 3rd, 2018 •
*1,2, *3, 2, 1
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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Her, beskriver vi en protokol for at opnå lipid droplet index (LD indeks) for at studere dynamikken i triglycerider i celler dyrkes i høj overførselshastighed eksperimenter. LD indeks assay er en nem og pålidelig metode, der bruger BODIPY 493/503. Denne analyse behøver ikke dispendious lipid udvinding eller mikroskopi analyse.

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Biokemi spørgsmål 134 Lipid indeks lipid dråber basidiomycete fluorescens opsving BODIPY 493/503 Ustilago maydis
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