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Journal / Biochemistry / Lipide Index bepaling door vloeibare…
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Biochemistry

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JoVE Journal Biochemistry
Lipide Index bepaling door vloeibare fluorescentie herstel in de schimmel pathogeen Ustilago Maydis
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Journal (Biochemistry)
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A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
Journal (Biochemistry)
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
 
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Lipide Index bepaling door vloeibare fluorescentie herstel in de schimmel pathogeen Ustilago Maydis

Article DOI: 10.3791/57279-v • 10:22 min • April 3rd, 2018
April 3rd, 2018 •
*1,2, *3, 2, 1
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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Hier beschrijven we een protocol om te verkrijgen van de lipide druppel index (LD index) om te bestuderen van de dynamiek van triacylglycerolen in cellen gekweekt in high-throughput experimenten. De LD index bepaling is een gemakkelijke en betrouwbare methode die gebruikmaakt van BODIPY 493/503. Deze test hoeft niet dispendious lipide extractie of microscopie analyse.

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Biochemie kwestie 134 lipide index lipide druppels lange fluorescentie recovery BODIPY 493/503 Ustilago maydis
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