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Journal / Genetics / Um método para estudar o polimorfismo C924T do…
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Genetics

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JoVE Journal Genetics
Um método para estudar o polimorfismo C924T do Gene do Receptor tromboxano A2
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Um método para estudar o polimorfismo C924T do Gene do Receptor tromboxano A2

Article DOI: 10.3791/57289-v • 07:00 min • April 1st, 2019
April 1st, 2019 •
1, 1, 2, 2, 2, 1, 3, 1, 2, 4, 1,2
1SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, 2Department of Medical, Oral and Biotechnological Sciences, University of Chieti, 3Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, 4CNR - Institute of Molecular Genetics, Section of Chieti

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No presente estudo, descrevemos uma metodologia para analisar o genótipo C924T. O protocolo consiste em três fases: extração de DNA, amplificação pela reação em cadeia da polimerase (PCR) e análise de polimorfismo de comprimento de fragmento de restrição (RFLP) em gel de agarose.

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Genética edição 146 expressão genética Biologia Molecular análise de enzimas de restrição Single Nucleotide Polymorphism genotipagem agregação plaquetária Atherothrombosis
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