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JoVE Journal Genetics
用 BIBAC 二元矢量生成单拷贝插入的转基因植物
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用 BIBAC 二元矢量生成单拷贝插入的转基因植物

Article DOI: 10.3791/57295-v • 12:08 min • March 28th, 2018
March 28th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1
1Swammerdam Institute for Life Sciences, University of Amsterdam

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使用 pBIBAC 的二进制向量, 可以生成具有完整单拷贝插入的转基因植物, 这是一个简单的过程。在这里, 提出了一系列的协议, 指导读者通过生成转基因拟南芥植物的过程, 并测试植物的完整性和复制数量的插入。

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遗传学 问题 133 二进制向量 BIBAC 网关兼容二进制向量 植物转化 单一整合 基因沉默 DNA 印迹 南方印迹
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