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Journal / Immunology and Infection / 复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14…
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Immunology and Infection

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JoVE Journal Immunology and Infection
复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
Journal (Immunology and Infection)
Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
Journal (Immunology and Infection)
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
 
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复制的有序, 非冗余图书馆的铜绿假单胞菌应变 PA14 座子插入突变体

Article DOI: 10.3791/57298-v • 11:35 min • May 4th, 2018
May 4th, 2018 •
*1, *1, 2, 1, 1, 2,3, 1,4, 1,4
1Department of Pediatrics, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, 2Department of Molecular Biology, Massachusetts General Hospital, 3Department of Genetics, Harvard Medical School, 4Department of Pediatrics, Harvard Medical School
* These authors contributed equally

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铜绿假单胞菌感染会导致易受害主机发病率显著。非冗余座子插入突变库的绿脓杆菌菌株 PA14, 指定为 PA14NR 集, 促进分析的基因功能在许多过程中。这里提出了一个协议, 以生成高质量的副本 PA14NR 集突变库。

Tags

免疫学和感染 问题 135 铜绿假单胞菌 水手座子 突变库 诱变 微生物学 机会感染 免疫学
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