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Journal / Developmental Biology / Mediada por CRISPR perda de função de análise no…
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Developmental Biology

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JoVE Journal Developmental Biology
Mediada por CRISPR perda de função de análise no grânulo cerebelar células usando no Utero Electroporation Gene transferência baseada
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Mediada por CRISPR perda de função de análise no grânulo cerebelar células usando no Utero Electroporation Gene transferência baseada

Article DOI: 10.3791/57311-v • 09:39 min • June 9th, 2018
June 9th, 2018 •
*1, *2, 2, 3,4,5, 1, 3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
* These authors contributed equally

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Estudos convencionais de perda-de-função de genes usando animais nocaute foram frequentemente dispendioso e demorado. Baseado em Electroporation mutagênese mediada por CRISPR somática é uma ferramenta poderosa entender gene funciona em vivo. Aqui, nós relatamos um método para analisar os fenótipos de nocaute nas células em proliferação do cerebelo.

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Biologia do desenvolvimento questão 136 entrega do gene alvo no utero electroporation cerebelo GNP Cre CRISPR/Cas9 desenvolvimento cerebral tumores cerebrais
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