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Journal / Bioengineering / Gel-seq: Un metodo per la preparazione di…
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Gel-seq: Un metodo per la preparazione di libreria simultanea di sequenziamento del DNA e RNA utilizzando matrici di idrogel

Article DOI: 10.3791/57315-v • 09:19 min • March 26th, 2018
March 26th, 2018 •
*1, *2, 2, 3, 2
1Department of Engineering, University of San Diego, 2Department of Bioengineering, University of California, San Diego, 3Department of Mechanical and Aerospace Engineering, University of California, San Diego
* These authors contributed equally

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Gel-seq consente ai ricercatori di preparare contemporaneamente le librerie per entrambi DNA - e RNA-seq trascurabile costo aggiunto a partire da 100-1000 cellule utilizzando un dispositivo semplice idrogel. Questa carta presenta un approccio dettagliato per la fabbricazione del dispositivo, nonché il protocollo biologico per generare librerie accoppiate.

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Bioingegneria problema 133 elettroforesi poliacrilammide idrogel sequenziamento di nuova generazione genoma trascrittoma libreria preparazione DNA RNA
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