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Journal / Biochemistry / Titrering ELISA som en metode til at bestemme…
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JoVE Journal Biochemistry
Titrering ELISA som en metode til at bestemme dissociationskonstant Receptor Ligand interaktion
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Titrering ELISA som en metode til at bestemme dissociationskonstant Receptor Ligand interaktion

Article DOI: 10.3791/57334-v • 12:38 min • February 15th, 2018
February 15th, 2018 •
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1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster

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En detaljeret protokol til at udføre en titrering ELISA er beskrevet. Derudover præsenteres en roman algoritme at evaluere titrering ringprøver og opnå en dissociationskonstant af bindingen af et opløseligt ligand til en mikrotiter plade-immobiliseret receptor.

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Biokemi sag 132 ELISA titrering assay dissociation konstant ikke-lineær regression receptor-ligand-interaktion rhodocetin
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