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Journal / Genetics / 增强基因组编辑与 Cas9 Ribonucleoprotein…
JoVE Journal
Genetics

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JoVE Journal Genetics
增强基因组编辑与 Cas9 Ribonucleoprotein 在不同的细胞和有机体
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增强基因组编辑与 Cas9 Ribonucleoprotein 在不同的细胞和有机体

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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利用预装 Cas9 ribonucleoprotein 复合体 (RNP) 是精确、高效的基因组编辑的有力方法。在这里, 我们强调它的效用横跨广泛的细胞和有机体, 包括主要的人类细胞和经典和新兴的模型有机体。

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遗传学 问题 135 CRISPR Cas9 gRNA RNP 基因组编辑 基因编辑 非目标 HDR HSPCs T 细胞,秀丽线虫 Parhyale hawaiensis
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