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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation av stort antal myeloida och dendritiska cellen prekursorer från murina benmärg med en roman Cell sortering strategi
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Journal (Immunology and Infection)
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
Journal (Immunology and Infection)
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
 
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Generation av stort antal myeloida och dendritiska cellen prekursorer från murina benmärg med en roman Cell sortering strategi

Article DOI: 10.3791/57365-v • 09:06 min • August 10th, 2018
August 10th, 2018 •
1, 1
1Department of Biological Sciences, Auburn University

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Här tillhandahåller vi en metod för att identifiera och isolera stort antal GM-CSF driven myeloida celler med hög hastighet cell sortering. Fem distinkta populationer (gemensamma myeloida, granulocyt/makrofag stamfäder, monocyter, monocyt-derived makrofager och monocyt-derived DCs) kan identifieras baserat på Ly6C och CD115 uttryck.

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Immunologi och infektion fråga 138 dendritiska cellen utveckling GM-SCF gemensamma myeloida stamceller granulocyt-makrofag stamceller monocyt monocyt-derived dendritiska cellen monocyt-derived makrofag hög hastighet cell sortering
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