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Journal / Cancer Research / Høj-følsomhed påvisning af Micrometastases…
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JoVE Journal Cancer Research
Høj-følsomhed påvisning af Micrometastases genereret af normal god landbrugspraksis Lentivirus-transduced Organoids kulturperler fra en Patient-afledte tyktarmen tumoren
Application of the En Bloc Concept Combined with Anatomic Resection in Laparoscopic Hepatectomy
Journal (Cancer Research)
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A Permanent Window for Investigating Cancer Metastasis to the Lung
Journal (Cancer Research)
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Høj-følsomhed påvisning af Micrometastases genereret af normal god landbrugspraksis Lentivirus-transduced Organoids kulturperler fra en Patient-afledte tyktarmen tumoren

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

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For at tillade meget følsomme påvisning af den udbrede menneskelige kolorektal cancer (CRC) celler koloniserer væv, viser vi heri en protokol for effektiv transduktion af grøn fluorescerende proteiner (NGL) lentiviral partikler i PDX-afledte CRC organoid celler forud for deres injektion i modtagerens mus, med stereo-fluorescens mikroskopiske observation.

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Kræftforskning sag 136 menneskelige CRC patient-afledte tumor xenograft (PDXs) normal god landbrugspraksis-mærket PDX-afledte tumor organoids lentiviral transduktion micrometastasis 3D kultur
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