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JoVE Journal Cancer Research
Høy følsomhet påvisning av Micrometastases generert av GFP Lentivirus-transduced Organoids kultivert fra en pasient-avledede kolon svulst
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Høy følsomhet påvisning av Micrometastases generert av GFP Lentivirus-transduced Organoids kultivert fra en pasient-avledede kolon svulst

Article DOI: 10.3791/57374-v • 09:16 min • June 14th, 2018
June 14th, 2018 •
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

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For at svært følsom påvisning av formidle menneskelige kolorektal kreft (CRC) celler kolonisere vev, viser vi her en protokoll for effektiv signaltransduksjon grønne fluorescerende protein (GFP) lentiviral partikler i PDX-avledet CRC organoid celler før deres injeksjon i mottakerens mus, med stereo-fluorescens mikroskopiske observasjon.

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Kreftforskning problemet 136 menneskelige CRC pasient-avledede svulst xenograft (PDXs) GFP-merket PDX-avledet svulst organoids lentiviral signaltransduksjon micrometastasis 3D kultur
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