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JoVE Journal Bioengineering
Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array
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Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Article DOI: 10.3791/57377-v • 07:19 min • September 7th, 2018
September 7th, 2018 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1, 1, 1, 3, 2, 4, 1,5, 1,6, 1
1Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, 2Department of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 3Biomaterials Center for Regenerative Medical Engineering, Foundation for Advancement of International Science, 4Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 5Institute for Chemical Research, Kyoto University, 6Ecole Normale Supérieure

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This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.

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Multiplexed Artificial Cellular MicroEnvironment Array Stem Cell Engineering Cellular Microenvironment Cellular Phenotypes Cellular Function Conventional Method Micro-contact Plate 3D Images Nano Fiber Arrays Molds Microfluidic Structures 3D Printer Polymer Solutions Electro Spinning PMGI Liquid Tetrahydrofuran PS Solution GT Solution Magnetron Sputtering Machine Platinum Layer Polystyrene Base Plate
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