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Cânula de implantação para a Cisterna Magna de roedores
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Cânula de implantação para a Cisterna Magna de roedores

Article DOI: 10.3791/57378-v • 10:13 min • May 23rd, 2018
May 23rd, 2018 •
1, 1, 1, 1, 1,2, 3, 3,4, 1,3
1Center for Translational Neuromedicine, Division of Glial Therapeutics, University of Copenhagen, 2Department of Anesthesiology, Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics, University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine, Lund University

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Aqui descrevemos um protocolo para realizar a cateterização da cisterna magna (CMc), uma forma minimamente invasiva para entregar traçadores, substratos e moléculas sinalizadoras no fluido cerebrospinal (CSF). Combinado com diferentes modalidades de imagem, CMc permite sistema de glymphatic e avaliação de dinâmica do CSF, bem como a entrega de todo o cérebro de vários compostos.

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Neurociência questão 135 líquido cefalorraquidiano (LCR) fluido intersticial (ISF) espaço subaracnoideo sistema glymphatic cisterna magna (CM) canulação de cisterna magna (CMc) roedores ressonância magnética (MRI) epifluorescência microscopia 2-fóton
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