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Journal / Genetics / RNA Pull-down Procedure til identifikation af en…
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Genetics

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JoVE Journal Genetics
RNA Pull-down Procedure til identifikation af en længe ikke-kodende RNA RNA mål
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RNA Pull-down Procedure til identifikation af en længe ikke-kodende RNA RNA mål

Article DOI: 10.3791/57379-v • 09:36 min • April 10th, 2018
April 10th, 2018 •
1, 1, 1, 1, 1, 2, 1, 1
1CNRS, CRN2M-UMR7286, Faculté de Médecine Nord, Aix-Marseille Université, 2Plate-forme d'imagerie MRI, UMS3426, CNRS 141, Institut de Génétique Humaine

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Denne RNA pull-down metode giver mulighed for at identificere RNA mål for en lang tid ikke-kodende RNA (lncRNA). Baseret på hybridisering af hjemmelavet, designede anti-sense DNA oligonukleotid sonder bestemt til denne lncRNA i en passende fast væv eller cellelinie, tillader det effektivt opsamling af alle RNA mål af lncRNA.

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Genetik spørgsmål 134 længe ikke-kodende RNA lnc RNA RNA pull-down sonde design forudsagt RNA sekundær struktur netværket tværbindingsmidler DNA og RNA klipning
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