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Journal / Developmental Biology / बँटवारा के दौरान गुणसूत्र अलगाव की लाइव सेल…
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JoVE Journal Developmental Biology
बँटवारा के दौरान गुणसूत्र अलगाव की लाइव सेल इमेजिंग
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बँटवारा के दौरान गुणसूत्र अलगाव की लाइव सेल इमेजिंग

Article DOI: 10.3791/57389-v • 06:39 min • March 14th, 2018
March 14th, 2018 •
1, 2, 1
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research, US Food and Drug Administration

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इस प्रोटोकॉल का वर्णन एक आसान और सुविधाजनक विधि लेबल और Histone2B-GFP BacMam 2.0 लेबल और एक कताई डिस्क फोकल माइक्रोस्कोपी प्रणाली का उपयोग कर mitotic कोशिकाओं में रहते गुणसूत्रों कल्पना ।

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विकास जीवविज्ञान अंक 133 गुणसूत्र अलगाव सेल चक्र नियंत्रण धुरी विधानसभा चौकी लाइव सेल इमेजिंग nocodazole माउस भ्रूण fibroblasts प्रोटीन फॉस्फेट 2a Histone2B-GFP BacMam 2.0
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