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JoVE Journal
Genetics

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405 Nm 激光微辐照对 DNA 损伤的蛋白质招募研究
 
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405 Nm 激光微辐照对 DNA 损伤的蛋白质招募研究

Article DOI: 10.3791/57410-v 12:29 min March 20th, 2018
March 20th, 2018

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Summary

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研究 DNA 损伤修复动力学需要一个系统来诱发定义的亚核区域的病变。我们描述了一个方法来创建本地化的双绞合, 使用激光扫描共聚焦显微镜配备405纳米激光, 并提供自动化程序, 以量化的修复因素在这些病变的动态。

Tags

遗传学 问题 133 dna 损伤 双链 dna 断裂 微辐照 共焦显微术 免疫荧光 实时成像 自动图像分析
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