Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / En enkel, hurtig og kvantitative analysen til…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
En enkel, hurtig og kvantitative analysen til foranstaltning reparation af DNA-protein krydsbindinger på plasmider transfekteret i pattedyrceller
In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
Journal (Genetics)
In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
Journal (Genetics)
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
 
Click here for the English version

En enkel, hurtig og kvantitative analysen til foranstaltning reparation af DNA-protein krydsbindinger på plasmider transfekteret i pattedyrceller

Article DOI: 10.3791/57413-v • 11:58 min • March 5th, 2018
March 5th, 2018 •
1, 1
1Department of Pharmacology, University of Minnesota

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Målet med denne protokol er at kvantificere reparation af definerede DNA-protein krydsbindinger på plasmid DNA. Lesioned plasmider er transfekteret ind i modtagerens pattedyr cellelinjer og lav-Molekylær vægt høstet på flere gang point efter Transfektion. DNA reparation kinetik er kvantificeret benytter strand-specifikke primer forlængelse efterfulgt af qPCR.

Tags

Genetik sag 133 DNA-protein krydsbindinger DNA reparation kvantitative polymerase kædereaktion 8-oxoguanine human oxoguanine glycosylase Taq-polymerase
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter