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Journal / Immunology and Infection / Mus - og menneske-avledet primære mage Epithelial …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Mus - og menneske-avledet primære mage Epithelial Monolayer kultur for studier av gjenfødelse
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Mus - og menneske-avledet primære mage Epithelial Monolayer kultur for studier av gjenfødelse

Article DOI: 10.3791/57435-v • 11:48 min • May 7th, 2018
May 7th, 2018 •
1, 2, 1, 3, 1
1Department of Pharmacology and Systems Physiology, University of Cincinnati, 2Department of Cell & Developmental Biology, University of Michigan, 3Department of Molecular Genetics, Biochemistry & Microbiology, University of Cincinnati

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Her beskriver vi en protokoll for å etablere og dyrking menneske - og mus-avledet 3-dimensjonale (3D) gastrisk organoids og metoden for overføring av 3D organoids til en 2-dimensjonal monolayer. Bruk av mage epithelial monolayer som en roman scratch-sår analysen for regenerering studier er også beskrevet.

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Immunologi og infeksjon problemet 135 scratch-sår analysen mage organoids stamceller reparasjon gjenfødelse epithelial monolayer
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