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JoVE Journal
Immunology and Infection

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泡沫病毒 Intasomes 原型的组装与纯化
 
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泡沫病毒 Intasomes 原型的组装与纯化

Article DOI: 10.3791/57453-v 10:20 min March 19th, 2018
March 19th, 2018

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Summary

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重组逆转录病毒整合酶和 dna 寡聚物模仿病毒 dna 末端可以形成一个酶活跃的复杂称为 intasome。Intasomes 可用于生物化学、结构和动力学研究。本协议详细介绍了如何组装和纯化原型泡沫病毒 intasomes。

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免疫学和感染 133 逆转录病毒 原型泡沫病毒 Intasome 整合酶 蛋白质复合体组装 蛋白质复合纯化
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