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Immunology and Infection

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JoVE Journal Immunology and Infection
Vergadering en zuivering van Prototype schuimend Virus Intasomes
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Vergadering en zuivering van Prototype schuimend Virus Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Recombinante retrovirale integrase en DNA oligomeren nabootsen van virale DNA uiteinden kunnen een enzymatisch actieve complex bekend als een intasome vormen. Intasomes kan worden gebruikt voor biochemische, structurele en kinetische studies. Dit protocol detailleert hoe te monteren en te zuiveren van prototype schuimend virus intasomes.

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Immunologie en infectie uitgeven 133 Retrovirus Prototype schuimend Virus Intasome Integrase eiwit Complex vergadering complexe eiwitreiniging
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