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Immunology and Infection

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JoVE Journal Immunology and Infection
Assemblaggio e purificazione del prototipo Virus schiumoso Intasomes
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Assemblaggio e purificazione del prototipo Virus schiumoso Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Integrasi retrovirale recombinante e oligomeri del DNA che imita le estremità del DNA virale possono formare un complesso enzimaticamente attivo conosciuto come un intasome. Intasomes possono essere utilizzati per studi biochimici, strutturali e cinetici. Questo protocollo i dettagli di come assemblare e purificare prototipo schiumoso virus intasomes.

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Immunologia e infezione numero 133 Retrovirus Virus schiumoso prototipo Intasome integrasi assiemi complessi della proteina complesso purificazione della proteina
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