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Immunology and Infection

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JoVE Journal Immunology and Infection
Montering og rensing av Prototype skummende Virus Intasomes
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Montering og rensing av Prototype skummende Virus Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Rekombinant retroviral integrase og DNA oligomers etterligne viral DNA ender kan danne en enzymatisk aktive komplekset kalles en intasome. Intasomes kan brukes for biokjemiske, strukturelle og kinetisk studier. Denne protokollen detaljer kursen og rense prototype skummende virus intasomes.

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Immunologi og infeksjon utstede 133 Retrovirus Prototype skummende Virus Intasome Integrase Protein kompleks sammenstilling Protein kompleks rensing
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