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JoVE Journal Immunology and Infection
Asamblea y purificación del prototipo Virus espumoso de los Intasomes
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Asamblea y purificación del prototipo Virus espumoso de los Intasomes

Article DOI: 10.3791/57453-v • 10:20 min • March 19th, 2018
March 19th, 2018 •
1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Integrasa de retrovirus recombinante y oligómeros de ADN mímico extremos de DNA virales pueden formar un complejo enzimático activo conocido como un intasome. Intasomes puede ser usada para estudios bioquímicos, estructurales y cinéticos. Este protocolo detalla cómo montar y purificar el prototipo virus espumoso de los intasomes.

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Inmunología e infección número 133 Retrovirus Virus espumoso de los prototipo Intasome integrasa conjunto complejo de proteínas purificación de complejos de proteínas
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