Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / CRISPR-Mediated Reorganization of Chromatin Loop…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
CRISPR-Mediated Reorganization of Chromatin Loop Structure
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
Journal (Genetics)
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Journal (Genetics)
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
 

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Article DOI: 10.3791/57457-v • 09:20 min • September 14th, 2018
September 14th, 2018 •
*1,2, *1, 1, 3, 4, 5, 1, 1, 1, 5, 3, 1,2,6
1Department of Dermatology, Program in Epithelial Biology, Stanford University School of Medicine, 2Program in Cancer Biology, Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology, Stanford University School of Medicine, 4Department of Biology, Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
* These authors contributed equally

Chapters

Summary
Automatic Translation

Chromatin looping plays a significant role in gene regulation; however, there have been no technological advances that allow for selective and reversible modification of chromatin loops. Here we describe a powerful system for chromatin loop re-organization using CRISPR-dCas9 (CLOuD9), demonstrated to selectively and reversibly modulate gene expression at targeted loci.

Tags

CRISPR-mediated Chromatin Loop Structure Gene Expression Chromatin Organization Transcriptional Dynamics Alter Chromosomal Architecture Long-range Chromatin Looping Reverse Chromatin Context Non-toxic Dimerizer Orthogonal Species Of Dcas9 Wide Applicability CRISPR-guide RNA Sequences Cell Cultures Plasmid Maps Lentiviral CRISPR Plasmid BsmB1 Alkaline Phosphatase Digestion Buffer DTT
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter