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JoVE Journal
Cancer Research

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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究
 
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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究

Article DOI: 10.3791/57460-v 08:30 min October 9th, 2018
October 9th, 2018

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Summary

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免疫分型小鼠原位 PDAC homografts 的实验程序旨在分析肿瘤免疫微环境。肿瘤通过手术原位植入。200–600毫米3的肿瘤被收获和分离, 以制备单细胞悬浮液, 然后使用不同的荧光标记抗体进行多免疫标记物的分析。

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癌症研究 问题 140 动物肿瘤模型 免疫肿瘤学 肿瘤移植 肿瘤组织离解 资产管制系统 直到 PDAC
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