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Journal / Cancer Research / Immunophenotyping di Orthotopic omotrapianto…
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Cancer Research

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JoVE Journal Cancer Research
Immunophenotyping di Orthotopic omotrapianto (Syngeneic) dell'Adenocarcinoma Ductal pancreatico murino primario KPC tramite flusso Cytometry
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Journal (Cancer Research)
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
Journal (Cancer Research)
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
 
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Immunophenotyping di Orthotopic omotrapianto (Syngeneic) dell'Adenocarcinoma Ductal pancreatico murino primario KPC tramite flusso Cytometry

Article DOI: 10.3791/57460-v • 08:30 min • October 9th, 2018
October 9th, 2018 •
*1,2, *1, 1, 1, 1, 1, 2, 1,2
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University
* These authors contributed equally

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La procedura sperimentale su immunophenotyping di omotrapianti PDAC murino ortotopico mira a profilatura immuno-microambiente tumorale. I tumori sono orthotopically impiantato tramite intervento chirurgico. Tumori di 200 – 600 mm3 dimensioni sono state raccolte e dissociati per preparare sospensioni unicellulari, seguite da analisi dell'indicatore multi-immuni FACS usando diversi anticorpi fluorescente etichettati.

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Ricerca sul cancro numero 140 modello animale del tumore Immuno-oncologia trapianto del tumore tumore del tessuto dissociazione FACS TIL TAM PDAC
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