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Journal / Cancer Research / Immunofænotypning af Orthotopic Homograft…
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JoVE Journal Cancer Research
Immunofænotypning af Orthotopic Homograft (Syngeneic) af Murine primære KPC pancreas duktalt adenokarcinom ved flowcytometri
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Immunofænotypning af Orthotopic Homograft (Syngeneic) af Murine primære KPC pancreas duktalt adenokarcinom ved flowcytometri

Article DOI: 10.3791/57460-v • 08:30 min • October 9th, 2018
October 9th, 2018 •
*1,2, *1, 1, 1, 1, 1, 2, 1,2
1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University
* These authors contributed equally

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Forsøgsmetoden på Immunofænotypning af murine orthotopic PDAC homografts sigter profilering tumor immuno-mikromiljø. Tumorer er orthotopically implanteret via kirurgi. Tumorer i 200-600 mm3 i størrelse blev høstet og adskilles for at forberede encellede suspensioner, efterfulgt af flere immun markør FACS analyse ved hjælp af forskellige fluorescently-mærket antistoffer.

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Kræftforskning spørgsmål 140 animalsk Tumor Model Immuno-onkologi Tumor Transplantation Tumor væv Dissociation FACS TIL TAM PDAC
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