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Journal / Genetics / Sequenciação de MicroRNA retrospectiva:…
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Genetics

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JoVE Journal Genetics
Sequenciação de MicroRNA retrospectiva
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Sequenciação de MicroRNA retrospectiva: Biblioteca de DNA complementar preparação protocolo usando amostras de RNA de parafina fixada em formol

Article DOI: 10.3791/57471-v • 12:24 min • May 5th, 2018
May 5th, 2018 •
1,2,3, 1,2, 3, 4
1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

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Fixada em formol parafina espécimes representam uma fonte valiosa de biomarcadores moleculares de doenças humanas. Aqui nós apresentamos um protocolo de preparação de biblioteca de base laboratorial do cDNA, inicialmente projetado com RNA congelado fresco e otimizado para a análise de microRNAs arquivados de tecidos armazenados até 35 anos.

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Genética edição 135 parafina FFPE RNA o RNA degradado microRNA geração de sequenciamento biblioteca de cDNA quantificação amplificação por PCR ligadura de RNA T4 página degrada o RNA fixada em formol
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