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JoVE Journal Bioengineering
Protokol for mikroRNA overførsel i knoglemarv-afledte hæmatopoietisk stamceller til at aktivere celle Engineering kombineret med magnetisk målretning
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Protokol for mikroRNA overførsel i knoglemarv-afledte hæmatopoietisk stamceller til at aktivere celle Engineering kombineret med magnetisk målretning

Article DOI: 10.3791/57474-v • 11:37 min • June 18th, 2018
June 18th, 2018 •
*1,2, *1,2, *1, 1,2, 1,2
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University
* These authors contributed equally

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Denne protokol illustrerer en sikker og effektiv procedure for at ændre CD133+ hæmatopoietisk stamceller. Den præsenteres ikke-virale, magnetiske polyplex-baserede tilgang kan danne grundlag for optimering af terapeutisk stamceller følger så godt som overvågning administreret celle produkt via magnetisk resonans.

Tags

Bioteknologi sag 136 stamceller CD133 genetisk modifikation ikke-virale Transfektion mikroRNA magnetiske målretning magnetiske nanopartikler polyethylenimine
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